期刊
SMALL
卷 6, 期 7, 页码 832-842出版社
WILEY-BLACKWELL
DOI: 10.1002/smll.200902084
关键词
biomedical materials; cells; cytotoxicity; magnetic materials; nanoparticles
类别
资金
- Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen)
- IWT (StrategischBasisOnderzoek) [80017]
Iron oxide nanoparticle internalization exerts detrimental effects on cell physiology for a variety of particles, hut little is known about the mechanism involved. The effects of high intracellular levels of four types of iron oxide particles (Resovist, Endorem, very small organic particles, and magneto-liposotnes (MLs)) on the viability and physiology of murine C17.2 neural progenitor cells and human blood outgrowth. endothelial cells are reported. The particles diminish cellular proliferation and affect the actin cytoskeleton and microtubule network architectures as well as focal adhesion formation and maturation. The extent of the effects correlates with the intracellular concentration (= iron mass) of the particles, with the biggest effects for Resovist and MLs at the highest concentration (1000 mu g Fe mL(-1)). Similarly, the expression of focal adhesion kinase (FAK) and the amount of activated kinase (pY397-FAK) are affected. The data suggest that high levels of perinuclear localized iron oxide nanoparticles diminish the efficiency of protein expression and sterically hinder the mature actin fibers, and could have detrimental effects on cell migration and differentiation.
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