期刊
STANDARDS IN GENOMIC SCIENCES
卷 10, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s40793-015-0105-y
关键词
Thermofilum; Crenarchaeota; hyperthermophile; Kamchatka; Phylogeny; Polysaccharides hydrolysis
资金
- Russian Science Foundation (RSF) [14-24-00165]
- Russian Federal Targeted Program for Research and Development (FTP RD) grant [14.575.21.0036]
- FTP RD grant [RFMEFI57514X0108]
A strain of a hyperthermophilic filamentous archaeon was isolated from a sample of Kamchatka hot spring sediment. Isolate 1807-2 grew optimally at 85 degrees C, pH 6.0-6.5, the parameters being close to those at the sampling site. 16S rRNA gene sequence analysis placed the novel isolate in the crenarchaeal genus Thermofilum; Thermofilum pendens was its closest valid relative (95.7 % of sequence identity). Strain 1807-2 grew organothrophically using polysaccharides (starch and glucomannan), yeast extract or peptone as substrates. The addition of other crenarchaea culture broth filtrates was obligatory required for growth and could not be replaced by the addition of these organisms' cell wall fractions, as it was described for T. pendens. The genome of strain 1807-2 was sequenced using Illumina and PGM technologies. The average nucleotide identities between genome of strain 1807-2 and T. pendens strain HRK 5(T) and T. adornatus strain 1910b were 85 and 82 %, respectively. On the basis of 16S rRNA gene sequence phylogeny, ANI calculations and phenotypic differences we propose a novel species Thermofilum uzonense with the type strain 1807-2(T) (= DSM 28062(T) = JCM 19810(T)). Project information and genome sequence was deposited in Genbank under IDs PRJNA262459 and CP009961, respectively.
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