期刊
SHOCK
卷 35, 期 3, 页码 322-328出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/SHK.0b013e3181facf73
关键词
Myocardial dysfunction; LPS; berberine; yohimbine; inhibitor of nuclear factor kappa B alpha; apoptosis
资金
- National Natural Science Foundation of China [30670826, 30971191]
- Science and Technology Foundation from Ministry of Education of the People's Republic of China [207140]
- Guangdong Natural Science Foundation [05103294]
- Guangdong Science and Technology Projects [2008B030301352]
Myocardial dysfunction is a common complication in sepsis and significantly contributes to the mortality of patients with septic shock. Our previous study demonstrated that pretreatment with berberine (Ber) protected against the lethality induced by LPS, which was enhanced by yohimbine, an alpha(2)-adrenergic receptor antagonist, and Ber combined with yohimbine also improved survival in mice subjected to cecal ligation and puncture. However, no studies have examined whether Ber and yohimbine reduce LPS-induced myocardial dysfunction. Here, we report that pretreatment with Ber, Ber combined with yohimbine, or yohimbine significantly reduced LPS-induced cardiac dysfunction in mice. LPS-provoked cardiac apoptosis, I-kappa B alpha phosphorylation, IL-1 beta, TNF-alpha, and NO production were attenuated by pretreatment with Ber and/or yohimbine, whereas cardiac Toll-like receptor 4 mRNA expression, malondialdehyde content, and superoxide dismutase activity were not affected. These data demonstrate for the first time that pretreatment with Ber and/or yohimbine prevents LPS-induced myocardial dysfunction in mice through inhibiting myocardial apoptosis, cardiac I-kappa B alpha phosphorylation, and TNF-alpha, IL-1 beta, and NO production, suggesting that activation of alpha(2)-adrenergic receptor in vivo may be responsible at least in part for LPS-induced cardiac dysfunction, and Ber in combination with yohimbine may be a potential agent for preventing cardiac dysfunction during sepsis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据