期刊
SEPARATION AND PURIFICATION TECHNOLOGY
卷 108, 期 -, 页码 83-88出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.seppur.2013.02.020
关键词
Concanavalin A; Iron oxide nanoparticles; Glycoprotein; Lactoferrin; Magnetic separation
资金
- National Science Council of the Republic of China [NSC 99-2120-M-006-007, NSC 100-2120-M-006-004]
Concanavalin A (Con A) was successfully bound onto Fe3O4 magnetic nanoparticles (MNPs) via carbodiimide activation as a magnetic nano-adsorbent for glycoprotein separation. The resulting Con A-bound Fe3O4 MNPs were nearly superparamagnetic with a mean diameter of 11.74 +/- 3.86 nm. The size and structure of Fe3O4 MNPs had no significant changes after Con A binding. The binding of Con A on the surface of Fe3O4 MNPs was confirmed by the analysis of Fourier transform infrared spectroscopy. From the thermogravimetric analysis and the concentration change of Con A in the binding solution, the weight percentage of Con A in the typical Con A-bound Fe3O4 MNPs was determined to be about 10 wt%. Furthermore, the Con A-bound Fe3O4 MNPs were found to be quite efficient for the fast adsorption of lactoferrin. The equilibrium was achieved within 5 min and the adsorption data for lactoferrin obeyed the Langmuir isotherm equation. At pH 7 and 25 degrees C, the maximum adsorption capacity and equilibrium constant were 59.2 mg/g and 0.0103 L/mg, respectively. In addition, by SDS-polyacrylamide gel electrophoresis, the capability of Con A-bound Fe3O4 MNPs for the selective adsorption of lactoferrin was further confirmed. (C) 2013 Elsevier B.V. All rights reserved.
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