期刊
SEPARATION AND PURIFICATION TECHNOLOGY
卷 62, 期 3, 页码 702-708出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.seppur.2008.03.028
关键词
thaumatin; aqueous two-phase system; polyethylene glycol/sodium sulfate system; two-level fractional factorial design; response surface methodology
资金
- IRPA
- MOSTI
The work attempts to study and optimize aqueous two-phase system (ATPS) made of polyethylene glycol (PEG)/sodium sulfate and PEG/potassium phosphate. Five factors (PEG's molecular weight, PEG's concentration, pH, concentration of sodium chloride and phase-forming salt) affecting the thaumatin partitioning were studied. A two-level fractional factorial was initially carried out. The statistical analysis showed that for both the systems, the phase-forming salt concentration and the concentration of sodium chloride significantly affects the K value for thaumatin partitioning. However, the performance of PEG/sodium sulfate system was generally better than the PEG/potassium phosphate system. Hence a detail study was carried out on the PEG/sodium sulfate system using central composite rotatable design (CCRD) in response surface methodology (RSM). it was observed that high salt concentration increased the K value by sevenfold and the PEG molecular weight was not a significant factor. The optimal condition gave a K value for thaumatin partitioning of 24.15 with a yield of 96.02%. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.
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