期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 279, 期 -, 页码 87-94出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.09.112
关键词
S. aureus; WGA; Magnetic separation; Gold nanoparticles; Blood; Sepsis diagnosis
资金
- Research Foundation from State Key Laboratory of Food Science and Technology, Nanchang University, China [SKLF-ZZB-201720]
- Educational Commission of Jiangxi Province, China [GJJ150014]
A novel 2-step lectin-magnetic separation (LMS) method combined with gold nanoparticle (AuNPs)-based colorimetric system for specifically and quantitatively detecting S. aureus in blood samples was established. In this study, wheat germ agglutinin lectin from Triticum vulgaris (wheat) (WGA) was applied for the first time as a recognition agent in a 2-step magnetic separation method using magnetic nanoparticles (MNPs) for effective and low-cost bacteria enrichment. A quantitative detection strategy based on the long dsDNA amplicons stabilized with AuNPs against salt-induced aggregation was investigated. The 2-step LMS method resulted in a high enrichment efficiency even at low concentrations (10(0)-10(2) CFU/mL) of S. aureus in pure culture and blood samples. The AuNPs-based colorimetric system was applied for quantitative detection of S. aureus with a limit of detection (LOD) at 3.5 x 10(0) CFU/mL. The complete process starting with the blood samples treatment, 2-step LMS, and AuNPs-based colorimetric detection took only 7h. The low cost and sensitive 2-step LMS combined with AuNPs-based colorimetric detection holds considerable potential for early sepsis diagnosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据