期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 276, 期 -, 页码 397-403出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.08.134
关键词
Fluorescent probes; Nitroreductase; Selectivity; Tumor cells; Bioimaging
资金
- NSF of China [21602139]
- Science & Technology Innovation Commission of Shenzhen [JCYJ20160520161235957]
The expression level of nitroreductase (NTR) is directly associated with cellular hypoxia states, which plays an important role in cancer invasion, metastasis, and resistance to treatment. Therefore, developing a facile and effective method for NTR detection is of great importance for improving anticancer treatment efficacies. To date, fluorescence method has received extensive attention for NTR detection due to its non-invasion, high sensitivity, and high spatial-temporal resolution features. However, most of fluorescent probes suffered from sophisticated systhesis procedure and poor biocompatibility, which would severely limit their practical applications especially for bioimaging. Herein, we repoted a novel chalcone-based fluorescence probe with simple preparation procedure and excellent biocompatibility for detection of NTR. The probe exhibits a highly selective fluorescence response towards NTR and the detection limit reaches 27 ng/mL. The specific fluorescence response can be ascribed to the NTR-catalyzed nitrobenzyl reduction mechanism, which has been verified by using fluorescence spectra, fluorescence lifetime, mass spectra, and inhibition experiments. Notably, this method successfully achieves hypoxia imaging in tumor cells, providing a useful tool for hypoxia pre-diagnosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据