期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 277, 期 -, 页码 388-393出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.09.028
关键词
Photoluminescence enhancement; Aggregation induced emission; AuAg nanoclusters; Label-free detection; Fluorescent sensor; Acinetobacter baumannii
资金
- National Key Research and Development Program of China [2017YFA0205300]
- National Natural Science Foundation of China [21675023, 91753106, 81325011]
- Scientific Research Foundation of Graduate School of Southeast University [YBJJ1829]
- Fundamental Research Funds for the Central Universities
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX17_0156]
The development of time-saving analytical methods for detection of Acinetobacter baumannii is of paramount importance. In this work, we have developed a two-step strategy to prepare highly photoluminescent AuAg nanoclusters (NCs) by using Ag as linkers to connect the Au tthiol motifs forming Au /Ag tthiol motifs on the tailored thiolated Au NCs, resulting in a significant photoluminescence enhancement. The strong photoluminescence of the as-synthesized AuAg bimetallic NCs can be rapidly and selectively quenched by A. baumannii due to its induced agglomeration of AuAg bimetallic NCs, which allows this fluorescent material to act as a label free A. baumannii sensor in aqueous solution. A linear relationship was demonstrated between the fluorescence intensity of the thiolated AuAg bimetallic NCs and the concentration of A. baumannii, in the range of 1 x 104 5 x 107 colony forming unit (CFU) /mL with a limit of detection of 2.3 x 103 CFU/mL. The A. baumannii content in sputum was also analyzed. As far as we know, this is the first report of a fluorescent sensor for the detection of A. baumannii. This approach represents a rapidly alternative method for the analysis of sputum A. baumannii in clinical diagnosis.
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