期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 203, 期 -, 页码 833-847出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2014.07.045
关键词
Fluorescent chemodosimeter; Cyanide; Phenazine-cyanine; Cell imaging; Near-infrareda
资金
- NSFC/China [21372082, 2116110444, 91233207, 21172073]
- National Basic Research 973 Program [2013CB733700]
Two chemodosimeters PDMI and PMI for cyanide detection were designed and synthesized based onphenazinecyanine dyes with N-methyl indolium group as receptor unit. According to the specific reactivity of indolium C-N+ bond against cyanide anion, both of them featured high sensitivity with detectionlimit of 1.4 mu M and 200 nM, respectively, and high selectivity against other anions. The quenching effecton phenazine-cyanine fluorophore by strong intramolecular charge transfer (ICT) from phenazine donorto indolium receptor made both PDMI and PMI non-emissive at the original state. After addition ofcyanide, the ICT effect decreased and vanished leading to dramatic off-on fluorescence enhancement. PDMI which proceeded bilateral electrophilic reaction toward cyanide anion provided an emission signal at 580 nm in HEPES buffer with naked-eye detectable color change. Probe PMI utilized an unreactiveformyl group instead of one reactive N-methyl indolium group as the electron-withdrawing component. Due to the unilateral recognition process for cyanide the ICT orientation of PMI was redirectedthus exhibited fluorescence enhancement with maximum emission at 630 nm. Meanwhile, PMI wasapplied for monitoring intracellular cyanide in Hela cells and proved to achieve off-on fluorescentsignal confirmed by confocal laser scanning microscopic imaging. (C) 2014 Elsevier B. V. All rights reserved.
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