期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 196, 期 -, 页码 546-554出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2014.02.052
关键词
Fluorescent probe; Cysteine detection; Protein; HeLa cell; Fluorescent bioimaging
资金
- NSFC [21062022, 21262039]
- State Key Laboratory of Supramolecular Structure and Materials
- Natural Science Foundation of Jiangsu Province [BK2011408]
A novel chemodosimetric fluorescent probe 1 for biothiols was designed and synthesized based on benzothiazolium-quinoline hemicyanine dye with an acrylate group as a functional trigger moiety. Prober 1 exhibited high sensitivity and high selectivity for rapid detection of cysteine (Cys) over homocysteine and glutathione in aqueous environments. The maximal fluorescent response was immediately achieved within 1 min in the presence of only 1 equiv. of Cys, and the color change of the sensing process was discerned visually at the nanomole level. The exceptional high reactivity and specific response of probe 1 for Cys were promoted by the stabilization of the sensing product phenolate anion and the differences in the kinetics of the sensing reaction. Moreover, probe 1 was employed successfully to detect the free Cys residues within protein and monitor Cys in living HeLa cells by fluorescence imaging. (C) 2014 Elsevier B.V. All rights reserved.
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