The application of natural drug-curcumin in the detection hypochlorous acid of real sample and its bioimaging

The detection property of natural drug-curcumin (1) was firstly reported by us. Experimental details showed 1 acts as an excellent UV-vis and fluorescent selectivity, sensitivity probe for the detection of hypochlorous acid, while other reactive oxygen species (ROS) and reactive nitrogen species (RNS) induced no changes in UV-vis spectra and fluorescence quench properties of I. A possible detection mechanism is that HOCl oxidized phenol of 1 into quinone resulting in a non-fluorescent curcumin derivative. Furthermore, the ability of probe to detect ClO- in living cells was also evaluated (HepG2 cells) via a cancelation of the fluorescence. Moreover, the application of 1 in quantitative analysis of hypochlorite in 84 disinfectant was also illustrated. (C) 2014 Elsevier B.V. All rights reserved.