期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 194, 期 -, 页码 290-295出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.12.098
关键词
ZnO nanorod; Cyclic voltammetry; Urease; Urea sensor
资金
- Priority Research Centers Program through the National Research Foundation (NRF) [2011-0031400]
- Korean Ministry of Education
- Pioneer Research Center Program through the NRF [2012-0001039]
- Korean Ministry of Science, ICT & Future Planning
Vertically aligned zinc oxide nanorods (ZnO NRs) were directly grown on Ag sputtered glass substrate by a low-temperature solution route. The fabricated urea sensor (glass/Ag/ZnO NRs/urease) showed a high sensitivity of 41.64 mu A/mM cm(2), wide linear range 0.001-24.0 mM, and low detection limit of 10 mu M. The low value of the Michaelis-Menten constant (K-M(app) = 0.328 mM) indicates the high affinity of urease towards the urea. The directly synthesized ZnO NRs on the electrode expressively increases NRs attachment and its specific surface area. These features further results in high enzyme loading and enhanced sensor efficiency. The urea sensor showed an excellent anti-interference ability against electroactive species. (C) 2013 Elsevier B.V. All rights reserved.
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