期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 192, 期 -, 页码 317-326出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.10.127
关键词
Electrochemical immunosensor; Magnetic silica nanoparticles; Graphene oxide; Click chemistry; Tumor marker
资金
- National Natural Science Foundation of China [21207048, 21277058]
- Technology Development Plan of Shandong Province, China [2011GGB01153]
- Natural Science Foundation of Shandong Province, China [ZR2011BQ019, ZR2012BZ002]
- State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences [KF2011-03]
A disposable electrochemical immunosensor was established for the detection of cancer antigen 153 (CA 153) using peroxidase-like magnetic silica nanoparticles/graphene oxide composites as labels and graphene oxide modified screen-printed carbon electrodes. The magnetic silica nanoparticles/graphene oxide composites were prepared via conjugating azide-functionalized magnetic silica nanoparticles to acetylene-functionalized graphene oxide by click chemistry with a copper-catalyzed 1,3-dipolar cycloaddition reaction. Structure characterization was obtained by means of atomic force microscope, X-ray diffraction and transmission electron microscope. The magnetic silica nanoparticles/graphene oxide composites showed great peroxidase-like activity. The CA 153 immunosensor was fabricated with sandwich method by immobilizing a monoclonal anti-CA 153 antibody on the graphene oxide attached on a screen-printed electrode, peroxidase-like magnetic silica nanoparticles/graphene oxide composites was used as signal label. This method was simple, inexpensive, highly sensitive, and selective for CA 153 detection with a proportional to the logarithm value of the CA 153 concentration from 10(-3) to 200 U/mL and the detection limit of 2.8 x 10(-4) U/mL. In addition, the assay was evaluated with CA 153 spiked serum samples and commercially available Electrochemiluminescent Analyzer obtained excellent correlation. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据