期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 190, 期 -, 页码 214-220出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.08.068
关键词
Bacteriophage; Peptide binders; SPR; Salmonella
资金
- European Union [273608]
- Marie Curie Fellowship from the EU
- National Science and Technology Development Agency (NSTDA)
- Department of Employment and Learning (DEL), ASSET project
Surface plasmon resonance (SPR)-based biosensor is a popular platform for real-time monitoring and sensitive detection for a myriad of targets. However, only a few studies have reported the use of bacteriophages as specific binders for SPR-based detection. This study aimed to demonstrate how filamentous M13 bacteriophages expressing 12-mer peptides can be employed in an SPR-based assay, using a Salmonella-specific bacteriophage as a model binder to detect the foodborne bacterium Salmonella. Several important factors (immobilization buffers and methods, and interaction buffers) for a successful bacteriophage-based SPR assay were optimized. As a result, a Salmonella-specific bacteriophage-based SPR assay was achieved, with very low cross reactivity with other non-target foodborne pathogens and detection limits of 8.0 x 10(7) and 1.3 x 10(7) CFU/mL for one-time and five-time immobilized sensors, respectively. This proof-of-concept study demonstrates the feasibility of using M13 bacteriophages expressing target-specific peptides as a binder in a rapid and label-free SPR assay for pathogen detection. (C) 2013 Elsevier B. V. All rights reserved.
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