期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 191, 期 -, 页码 600-604出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.10.025
关键词
Mercury; DNAzyme; Magnetic beads; T-Hg2+-T
资金
- NSFC [21177023, J1103303]
- Key Project of Chinese Ministry of Education [211084, 20123514110001]
- Science Foundation of Fujian Province of China [2013J01044, 2012J01035]
- National Key Technologies R&D Program of China during the 12th Five-Year Plan Period [2012BAD29806]
A simple and sensitive DNAzyme-based colorimetric sensor for detecting Hg2+ based on T-Hg2+-T base pair and magnetic beads (MBs) is proposed. In the presence of Hg2+, capture probes (CPs) immobilized on MBs are hybridized with the signal probes (SPs) consisting of G-quadruplex sequences with the help of T-Hg2+-T base pairs. The SPs are separated by MBs and used to bind hemin for the formation of catalytically active hemin/G-quadruplex DNAzymes, which catalyzes the H2O2-mediated oxidation of ABTS to produce a blue-green-colored product. A linear relationship between the absorbance and Hg2+ concentration is observed over a range of 10-200 nM with a limit of detection 5 nM. Taking advantage of MBs separation/collection process, the sensing system can be regenerated by using EDTA as the chelator. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据