期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 190, 期 -, 页码 685-693出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.09.062
关键词
Near-infrared dye; Viscosity probe; Fluorescence ratio; Fluorescence lifetime; Mitochondrial viscosity; Apoptosis
资金
- NSF of China [21136002, 21076032, 20923006]
- National Basic Research Program of China [2009CB724706, 2013CB733702]
- National High Technology Research and Development Program of China (863 Program) [2011AA02A105]
Viscosity strongly influences intracellular transportation of mass and signal, and although bulk viscosity can be well measured with macroscopic samples quantities, microscale measurements in live cells still remain a challenge. By introducing a CHO group into the meso-position of the pentamethine chain, a thiazole-Cy5 dye based two-photon fluorescence probe, Mito-V, was developed. It exhibited a selective response to solution viscosity by fluorescence ratio and lifetime methods. More importantly, Mito-V can localize in mitochondria and quantitatively image its viscosity changes by the dual fluorescence mode during the apoptosis. (c) 2013 Elsevier B. V. All rights reserved.
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