期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 188, 期 -, 页码 1101-1108出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.07.109
关键词
1-Butyl-3-methylimidazolium chloride; Catechol determination; Dihexadecylphosphate; Ionic liquid; Multi-walled carbon nanotubes; Tyrosinase biosensor
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Instituto Nacional de Ciencia e Tecnologia de Bioanalitica (INCTBio)
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
- Fundacao para a Ciencia e a Tecnologia (FCT), Portugal [PTDC/QUI-QUI/116091/2009]
- POCH
- POFC-QREN
- FSE
- European Community FEDER funds through the program COMPETE
- FCT project [PEst-C/EME/UI0285/2013]
- Fundação para a Ciência e a Tecnologia [PTDC/QUI-QUI/116091/2009] Funding Source: FCT
A glassy carbon electrode modified with functionalized multi-walled carbon nanotubes (MWCNT), 1-butyl-3-methylimidazolium chloride (ionic liquid = IL) and tyrosinase (Tyr) within a dihexadecylphosphate (DHP) film for the development of a biosensor is proposed. MWCNT, IL and Tyr were efficiently immobilized in the film using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) as crosslinking agents, and which was characterized by cyclic voltammetry (CV) in the presence of catechol. The IL-MWCNT nanocomposite showed good conductivity and biocompatibility with Tyr enzyme, since the biosensor presented biocatalytic activity toward the oxidation of catechol to o-quinone which was electrochemically reduced to catechol at a potential of 0.04 V. The analytical curve ranged from 4.9 x 10(-6) to 1.1 x 10(-3) mol L-1 with a detection limit of 5.8 x 10(-7) mol L-1. The developed Tyr-IL-MWCNT-DHP/GCE biosensor showed a wide linear range, good reproducibility, sensitivity and stability and the biosensor was successfully applied to the determination of catechol in natural water samples, with satisfactory results compared with a spectrophotometric method, at the 95% confidence level. (C) 2013 Elsevier B.V. All rights reserved.
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