期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 176, 期 -, 页码 118-123出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2012.09.110
关键词
LTCC; Polymerase chain reaction (PCR); Microfluidics; Bovine serum albumin (BSA); Taq polymerase; Inhibition
资金
- Environmental Protection Agency, Boise State University Sensor Program [CN X97031101-0]
The increasing use of low temperature co-fired ceramic (LTCC) for the fabrication of biological microfluidic devices necessitates further research on LTCC biocompatibility. In this study we explore the inhibitory effect of DuPont's 951 LTCC on Polymerase Chain Reaction (PCR), and demonstrate a novel mechanism to increase biocompatibility between LTCC and PCR with the addition of a common passivation substance, bovine serum albumin (BSA). We show that DuPont's 951 LTCC binds negatively charged proteins including BSA and ovalbumin (OVA). This is a significant discovery as proteins (enzymes) are an essential component of most biological reactions, and a frequent addition to microfluidic devices. A proposed model for LTCC inhibition of PCR by enzyme adsorption is presented. (C) 2012 Elsevier B.V. All rights reserved.
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