期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 177, 期 -, 页码 64-69出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2012.10.114
关键词
Lactose determination; Biosensor; Cellobiose dehydrogenase; Single-walled carbon nanotubes; Aryl diazonium salts
资金
- The Swedish Chemical Society
- Swedish Research Council
- Foundation for Research, Science and Technology of New Zealand [13838-NMTS-LVL]
- Nanometer Consortium at Lund University
- Austrian Science Foundation [L395-B11]
In this paper a new third-generation amperometric biosensor for lactose determination is described. The biosensor is based on the highly efficient direct electron transfer (DET) between cellobiose dehydrogenase (CDH) from Phanerochaete sordida (PsCDH) and single walled carbon nanotubes (SWCNT). The SWNCTs were surface modified with aryl diazonium salts of p-phenylenediamine (NH2-PD) and deposited on top of a glassy carbon (GC) electrode. The PsCDH NH2-PD/SWCNT-GC biosensor showed very efficient DET and exhibited an extraordinary high current density of 500 mu M cm(-2) in a 5 mM lactose solution at pH 3.5. The biosensor has a detection limit for lactose of 0.5 mu M, a large linear range from 1 to 150 mu M lactose and a high sensitivity (476.8 nA mu M-1 cm(-2)). It shows also a fast response time (4s), good reproducibility (RSD =1.75%) and good stability (half-life 12 days). In addition, it is easy, simple to manufacture, and cheap because a low amount of enzyme is required and highly selective, as no significant interference was observed. For these reasons, it can represent a valid alternative to HPLC measurements for lactose determination in milk and dairy products. (c) 2012 Elsevier B.V. All rights reserved.
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