期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 188, 期 -, 页码 1250-1254出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.08.027
关键词
Salmonella; Personal glucose meter; Magnetic nanoparticle clusters
资金
- Ministry of Food and Drug Safety [10162MFDS995]
- Ministry of Food & Drug Safety (MFDS), Republic of Korea [10162MFDS995, 10162기후식995-4206, 10162기후식995] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
We report a facile and sensitive method for the detection of Salmonella bacteria in milk using a personal glucose meter (PGM). Monoclonal antibody-functionalized magnetic nanoparticle clusters (MNCs) were used to capture Salmonella bacteria in milk, and MNC-Salmonella complexes were magnetically separated from the sample using a permanent magnet. The complexes were further conjugated to polyclonal antibody-functionalized invertase and dispersed in a 0.5 M sucrose solution. After the hydrolysis of sucrose to glucose and fructose, the concentration of glucose was measured using the PGM. The hydrolysis reaction was conducted at various temperatures, and the optimal temperature and activation energy were determined. The detection limit of Salmonella in milk was found to be 10 cfu/mL. (C) 2013 Elsevier B. V. All rights reserved.
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