期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 178, 期 -, 页码 525-531出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2012.12.108
关键词
Chemosensor; Benzoxazine; Biothiol; Ratiometric; Blue-shift
资金
- National Natural Science Foundation of China [51273136]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Aliphatic biothiols, including cysteine, homocysteine, and glutathione, are associated with a wide range of biological functions. Hence, the selective detection of intracellular thiols has significance for investigating cellular function. Ratiometric fluorescent probes are very useful in the biological sciences because they allow measurement at two emission wavelengths, providing a built-in correction for environmental effects. In this study, a benzoxazine-hemicyanine based probe (1) was synthesized and characterized. The probe shows quick colorimetric and ratiometric response toward biothiols in the presence of other biomolecules and it functions in a wide pH range exhibiting large shifts in both absorption and fluorescence spectra. The detection mechanism, which was induced by the addition of mercaptan toward the activated double bond in the probe, was verified using H-1 NMR and mass spectrometry analysis. The photophysical properties changes that occurred upon the reaction of the probe were found to be induced by the variation of intermolecular charge transfer, which was confirmed by theoretical calculations based on density functional theory (DFT). The fluorescence imaging of intracellular thiols was also examined with excellent performance. (c) 2013 Elsevier B.V. All rights reserved.
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