期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 188, 期 -, 页码 257-262出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.06.087
关键词
Oxygen sensor; Fluorescence; Two-photon excitation; Quenching; Nanosensor; Intracellular sensing
Polystyrene nanoparticles (PSNPs) with an average size of 85 nm and loaded with an oxygen-quenchable luminescent ruthenium complex were used to sense and image oxygen inside cells following 2-photon excitation (2-PE). The ruthenium probe possesses a large two-photon absorption cross-section, and 2-PE is achieved by irradiation in the near infrared with commercially available fs-pulsed laser systems. The luminescence of the dye-loaded PSNPs is strongly quenched by oxygen, and Stern-Volmer plots are linear for both conventional single-photon excitation (1-PE) and for 2-PE. The particles do not show any significant cytotoxicity below a threshold concentration of 5 mu g/mL and are readily taken up by mammalian cells (MCF-7), presumably via membrane mediated pathways. Thus, the PSNPs promise to be well suited to image the oxygen distribution in living cells and tissues. The 2-PE is considered to be advantageous over conventional imaging techniques because it works in the near-infrared where background absorption and luminescence of biomatter is much weaker than at excitation wavelengths below 600 nm. (C) 2013 Elsevier B.V. All rights reserved.
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