期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 183, 期 -, 页码 187-193出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.03.139
关键词
Voltammetric immunosensor; Human enterovirus 71; Label-free; Gold nanoparticles; Poly(o-phenylenediamine)
资金
- National Basic Research Program of China [2011CB933502]
- International S&T Cooperation Projects of China [2010DFA42060]
- Natural Science Foundation of China [21275023, 21121091]
- Natural Science Foundation of Jiangsu Province [BK2012593]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
The detection of human enterovirus 71 (EV 71), an important etiological agent of foot, hand and mouth disease, by a hybrid voltammetric immunosensor was proposed in this paper for the first time. Poly(o-phenylenediamine) (PoPD) film was formed on a glassy carbon electrode (GCE) by utilizing electropolymerization, which yielded an interface with amino groups for the subsequent assembly of gold nanoparticles (AuNPs). The electrochemical characteristics of the immunosensor were investigated by cyclic voltammetry (CV). The parameters influencing the performance of the resulting immunosensor were studied in detail. The current response of the proposed immunosensor decreased linearly in the EV 71 concentration range from 0.1 to 80 ng mL(-1) with a detection limit of 0.04 ng mL(-1) (signal-to-noise ratio of 3). Due to its simple preparation, good reproducibility and long-time stability, the proposed voltammetric immunosensor will be promising in the point-of-care diagnostics application of clinical screening of multiple diseases. (C) 2013 Elsevier B.V. All rights reserved.
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