期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 181, 期 -, 页码 319-325出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.02.026
关键词
Methylene blue; DNA hybridization; Ag/NaA zeolite; Modified carbon paste electrode; DNA biosensor
In this research, we reported a method for synthesis of NaA zeolite. NaA zeolite has been successfully prepared within 30 min by microwave heating without using any conventional hydrothermal treatment and due to the exchange process the silver-loaded NaA microsized zeolites was produced. The synthesized zeolite was characterized using X-ray diffraction, scanning electronic microscopy and FT-IR techniques. The modified carbon paste electrode was prepared by incorporation of Ag(I) zeolite in the carbon paste matrix. Differential pulse voltammetry (DPV) was employed for development of electrochemical DNA hybridization biosensors based on Ag/NaA zeolite modified carbon paste electrode (Ag/ZMCPE) and methylene blue (MB) as electroactive label was used. The sensors rely on immobilization of a 15-mer single stranded oligonucleotide probe for detection of target DNA, as a model. The hybridization event was evaluated by DPV. Moreover, Ag/ZMCPE showed better advantages than NaA zeolite modified carbon paste electrode (ZMCPE). Under optimized experimental conditions, limit of detection was calculated 4.00 x 10(-12) M. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据