期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 178, 期 -, 页码 606-614出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2012.12.077
关键词
Glutamate; Biosensor; o-Phenylenediamine; Nafion; Selectivity; Stability
资金
- Northern Brainwave Appeal
Glutamate mediates most of the excitatory synaptic transmission in the brain, and its abnormal regulation is considered a key factor underlying the appearance and progression of many neurodegenerative and psychiatric diseases. In this work, a microdisc-based amperometric biosensor for glutamate detection with highly enhanced selectivity and good stability is proposed. The biosensor utilizes the enzyme glutamate oxidase which was dip-coated onto 125 mu m diameter platinum discs. To improve selectivity, phosphatidylethanolamine was pre-coated prior to enzyme deposition, and electropolymerization of o-phenylenediamine was performed to entrap the enzyme within a polymer matrix. A variety of coating configurations were tested in order to optimize biosensor performance. For stability measurements, biosensors were biased continuously and calibration curves calculated each day for a period of 5-6 days. The optimized biosensors exhibited very high sensitivity (71 +/- 1 mA M-1 cm(-2)), low detection limit of similar to 2.5 mu M glutamate, selectivity (over 87% against ascorbic acid), very good temporal stability during continuous use, and a response time of <5 s. These biosensors are therefore good candidates for further development as devices for continuous monitoring during traumatic brain injury or neurosurgery. (c) 2013 Elsevier B.V. All rights reserved.
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