期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 188, 期 -, 页码 417-424出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.07.058
关键词
Electrochemiluminescence immunosensor; Tumor markers; Microfluidic origami device; Point-of-care testing
资金
- Natural Science Research Foundation of China [21277058, 21175058]
- Natural Science Foundation of Shandong Province, China [ZR2012BZ002, ZR201113Q019]
- Technology Development Plan of Shandong Province, China [2011GGB01153, 2011GGX10319]
A highly sensitive electrochemiluminescence (ECL) immunosensor combined with a 3D origami device for detection of carcinoembryonic antigen (CEA) was developed based on novel porous silver nanoparticles modified paper working electrode (Ag-PWE) as sensor platform and CdTe quantum dots functionalized nanoporous silver (QDs/NPS) as signal amplification label. The microfluidic origami device was fabricated by directly screen-printed electrodes on wax-patterned cellulose paper in bulk. The Ag-PWE was fabricated by a seed-mediated growth approach and served as an effective matrix for antibody attachment with good bioactivity, and stability. The ECL nanoprobe (Ab2RD5/NPS) was designed by covalent assembly of signal antibodies (Ab(2)) on QDs/NPS. After a sandwich immunoreaction, the functionalized NPS labels were captured onto the Ag-PWE surface. On the basis of the considerably amplified ECL signal and sandwich-type format, the proposed method successfully fulfilled the highly sensitive detection of CEA with a linear range of 0.5 pg mL(-1) to 20 ng mL(-1) with a detection limit of 0.12 pg mL(-1). This facile origami ECL immunodevice exhibited high sensitivity, specificity and excellent performance in real human serum assay, and could be applied in point-of-care testing of other tumor markers for remote regions and developing countries. (C) 2013 Elsevier B.V. All rights reserved.
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