期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 176, 期 -, 页码 1-8出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2012.08.035
关键词
Microfluidic origami device; Screen-printed electrodes; Electrochemiluminescence immunosensor; Tumor markers; Point-of-care testing
资金
- Technology Development Plan of Shandong Province, China [2011GGB01153]
- Natural Science Research Foundation of China [21175058]
- Natural Science Foundation of Shandong Province, China [ZR2012BZ002, ZR2011BQ019]
In this paper, a 3D microfluidic origami device combined with electrochemiluminescence (ECL) immunosensor was introduced for sensitive point-of-care testing of carcinoma antigen 125 in clinical serum samples for the first time. This microfluidic origami device was fabricated by directly screen-printing carbon working, counter electrodes and Ag/AgCl reference electrode including their conductive pads on wax-patterned pure cellulose paper, and was activated by folding to form a 3D electrochemical cell. To construct a sensitivity-enhanced sandwich-type ECL immunosensor, gold nanoparticles (AuNPs) was synthesized to modify the working electrode to provide a good pathway of electron transfer and enhance the immobilized amount of capture antibody (McAb(1)), and luminol functionalized gold nanoparticles (Lu-AuNPs) was synthesized to label signal antibody (McAb(2)). With the aid of a facile device-holder, cyclic voltammetry was applied on microfluidic origami ECL immunodevice to trigger ECL. Under optimal conditions, this microfluidic origami ECL immunodevice provided a good linear response range from 0.01 to 100 U mL(-1) with a detection limit of 0.0074 U mL(-1). The proposed ECL immunodevice presented high sensitivity and stability, and could be applied in point-of-care testing of other tumor markers for remote regions and developing countries. (C) 2012 Elsevier B.V. All rights reserved.
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