期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 176, 期 -, 页码 253-257出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2012.10.052
关键词
Aptasensors; Colorimetric assay; Magnetic nanoparticles; Peroxidase-like activity
资金
- Korea Institute of Science and Technology (KIST)
We developed a simple colorimetric assay for the detection of metal ions based on the peroxidase-like activity of magnetic nanoparticles (MNPs) and their interactioin with target specific single-stranded DNA (ssDNA). It was already known that MNPs easily oxidize substrates in the presence of hydrogen peroxide same as a peroxidase. In this study, the target specific ssDNA was adsorbed on the positively charged surface of MNP, then the catalytic activity of MNPs was reduced by the screening effect of adsorbed ssDNA on the surface of MNPs to substrates. The peroxidase like activity of MNPs was recovered by the addition of targets because the target specific ssDNA were displaced from MNPs by the interaction between targets and their specific ssDNA. The degree of color change of mixture was proportional to the concentration of mercury ion in a range of 5-75 mu M. In control experiment, other metal ions did not induce the color change. (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据