期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 173, 期 -, 页码 847-851出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2012.07.114
关键词
Jaffe's reaction; Picric acid; Differential pulse technique; Indirect quantification; Non-modified electrodes; Selectivity
资金
- FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo) [2009/07859-1, 2011/11115-8, 2011/19903-5]
- CAPES
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) [470919/2011-6, 302700/2011-0]
The aim of this study is to develop a new enzymeless electroanalytical method for the indirect quantification of creatinine from urine sample. This method is based on the electrochemical monitoring of picrate anion reduction at a glassy carbon electrode in an alkaline medium before and after it has reacted with creatinine (Jaffe's reaction). By using the differential pulse voltammetry technique under the optimum experimental conditions (step potential, amplitude potential, reaction time, and temperature), a linear analytical curve was obtained for concentrations of creatinine ranging from 1 to 80 mu mol L-1, with a detection limit of 380 nmol L-1. This proposed method was used to measure creatinine in human urine without the interference of most common organic species normally present in biological fluids (e.g., uric acid, ascorbic acid, glucose, and phosphocreatinine). The results obtained using urine samples were highly similar to the results obtained using the reference spectrophotometric method (at a 95% confidence level). (C) 2012 Elsevier B.V. All rights reserved.
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