期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 168, 期 -, 页码 395-401出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2012.04.043
关键词
Electrochemical sensor; Molecularly imprinted polymers; Pyrrole; Bovine hemoglobin; Recognition
资金
- National Natural Science Foundation of China [21005002]
- Anhui Provincial Natural Science Foundation [11040606Q35]
- Anhui University Provincial Natural Science Foundation [KJ2010A138]
- Dr Start-up Foundation of the Anhui Normal University [160-750834]
A simple and efficient molecularly imprinted polymers (MIPs) based electrochemical sensor were proposed and prepared by electropolymerization of pyrrole in the presence of bovine hemoglobin (BHb) in an aqueous solution. The fabrication process of the sensor was characterized by differential pulse voltammetry and electrochemical impedance spectroscopy, in which [Fe(CN)(6)](3-)/[Fe(CN)(6)](4-) was used as an electrochemical active probe. The influence factors including electropolymerization cycles, scan rate, the concentration of pyrrole, and the extraction conditions were investigated in detailed. Under the optimized conditions, the experimental results showed that the MIPs based sensor possessed a fait rebinding dynamics and an excellent recognition capacity to BHb, compared to the other non-template proteins. Moreover, the prepared sensor also exhibited a dependent relationship between the concentration of template protein and peak current of [Fe(CN)(6)](3-)/[Fe(CN)(6)](4-). (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据