期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 171, 期 -, 页码 803-809出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2012.05.074
关键词
Electrochemiluminescence immunosensor; Carcinoembryonic antigen; Ru(bpy)(3)(2+)-graphene-Nafion composites; ECL quenching
资金
- National Natural Science Foundation of China [81072336]
- 973 Program [2010CB635116]
- Science and Technology Department of Zhejiang Province of China [2010C33004, 2011R405051]
- Ningbo Science and Technology Bureau [2010A610030, 2011050037]
- Ningbo University [xk111049]
- Scientific Research Foundation of Graduate School of Ningbo University
- K.C. Wong Magna Fund in Ningbo University
An ultrasensitive electrochemiluminescence (ECL) immunosensor for the detection of carcinoembryonic antigen (CEA) in serum and saliva was developed. Ru(bpy)(3)(2+)-graphene-Nafion composite was first coated on the glassy carbon electrode surface in this ECL immunosensor. The sandwich-type immunoreactions between the first antibody and the second antibody bridged the donors Ru(bpy)(3)(2+) and acceptors QDs, which led to the occurrence of ECL quenching of Ru(bpy)(3)(2+) by QDs. Based on the efficient ECL quenching, ultrasensitive CEA detection was realized. Under optimal conditions, the ECL signal depended linearly on the logarithm of the CEA concentration within a range from 0.005 to 0.5 pg/mL, and the detection limit of CEA was 0.002 pg/mL. Moreover, the proposed ECL immunosensor provides a stable, specific, and highly sensitive immunoassay protocol for CEA detection at very low levels, which might hold a promise for clinical application. (C) 2012 Elsevier B.V. All rights reserved.
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