期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 154, 期 1, 页码 22-27出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2010.03.067
关键词
Microfluidics; Biosensors; DNA hybridization; Genotyping; Electrical detection
资金
- NHGRI NIH HHS [P01 HG000205-110004, P01 HG000205] Funding Source: Medline
Current methods used for detection of DNA hybridization involve the use of DNA microarrays which require overnight incubation times along with bulky and expensive fluorescent scanners. Here, we demonstrate electrical detection of DNA hybridization in an oligonucleotide functionalized microfluidic channel. We use microchannels functionalized with DNA probes integrated with electrodes for measuring conductance across the channel. As beads conjugated with the target DNA passing through the channel are captured on the surface, we are able to electrically detect changes in resistance due to bead capture. Our assay can be completed in less than an hour using less than a microliter of reagent, and has the potential for extensive multiplexing. Such a device can be useful as a handheld platform in a clinical setting where one would need to rapidly genotype a small number of genes rapidly. (C) 2010 Elsevier B.V. All rights reserved.
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