期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 159, 期 1, 页码 314-320出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2011.06.043
关键词
Quantum dot; DNA; On-chip sorter; Separation; Microfluidics; Thermo-reversible gelation polymer
资金
- Sentan
- JST
- Ministry of Education, Culture, Sports, Science and Technology of Japan [19206046, 20-710103]
- Japan Society for the Promotion of Science (JSPS)
- Japan Society for the Promotion of Science for Young Scientists [20-9830, 20-10635]
- Grants-in-Aid for Scientific Research [19206046, 23770189, 22020006] Funding Source: KAKEN
The fluorescence-activated cell sorter instrument has contributed significantly to life sciences. However, this instrument has limitations including the inability to detect and hence sort nanometer-sized particles such as quantum dots (Qdots) and virus particles. Here, a microfluidic device for analyzing and sorting nanometer-sized particles has been developed. To achieve sensitive detection, the sample flow was hydrodynamically sheathed and effectively excited with a focused laser beam. Flow control was performed by a sot-gel transition of a thermoreversible gelation polymer. This flow control approach enabled us to restrict the lateral diffusion of nanometer-sized particles and to sort the particles in similar to 10 mu m channels. Single Qdots with diameters of similar to 10 nm were detected at a linear flow velocity of about 4 mm/s, and the Qdots were successfully sorted with the sorting system. Using the developed sorter in an application, Qdot-labeled actin DNA was separated from unwanted glyceraldehyde-3-phosphate dehydrogenase DNA, and the purity of Qdot-labeled actin DNA increased following the sorting. This study represents the first example of active separation of biomolecules labeled with single 10 nm-sized particles of Qdots. (C) 2011 Elsevier B.V. All rights reserved.
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