期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 152, 期 1, 页码 49-55出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2010.09.051
关键词
Gold nanoparticles; Glucose oxidase; Eggshell membrane; Glucose biosensor
资金
- Research Grants Council of the Hong Kong Special Administrative Region, China [HKBU 200907]
- National Natural Science Foundation of China [20775050, 20927007]
This work reports the fabrication and application of a glucose biosensor based on the catalytic effect of gold nanoparticles (AuNPs) on enzymatic reaction for blood glucose determination. AuNPs were initially in situ synthesized on the surface of an eggshell membrane (ESM) which was subsequently immobilized with glucose oxidase (GO(x)) to produce a GO(x)-AuNPs/ESM. The GO(x)-AuNPs/ESM was positioned on the surface of an oxygen electrode to form a GO(x)-AuNPs/ESM glucose biosensor. The effects of pH, concentration of phosphate buffer solution and amount of GO(x) on the response of the GOx-AuNPs/ESM glucose biosensor were studied in detail. AuNPs on GO(x)/ESM can improve the calibration sensitivity (30% higher than GO(x)/ESM without AuNPs), stability (87.3% of its initial response to glucose after 10-week storage) and shortens the response time (< 30 s) of the glucose biosensor. The linear working range for the GO(x)-AuNPs/ESM glucose biosensor is 8.33 mu M to 0.966 mM glucose with a detection limit of 3.50 mu M (S/N= 3). The biosensor has been successfully applied to determine the glucose in human blood serum samples and the results compared well to a standard spectrophotometric method commonly used in hospitals. Our work demonstrates that the developed GO(x)-AuNPs/ESM glucose biosensor has potential in biomedical analysis. (c) 2010 Elsevier B.V. All rights reserved.
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