期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 157, 期 1, 页码 304-309出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2011.03.082
关键词
Surface plasmon resonance imaging (SPRi); Drug discovery; Quadruplex binding
资金
- French ANR [ANR-07-BLAN-0343-01]
- Genoptics Horiba-Scientific
- Agence Nationale de la Recherche (ANR) [ANR-07-BLAN-0343] Funding Source: Agence Nationale de la Recherche (ANR)
Surface plasmon resonance imaging (SPRi) is a label free technology for biomolecular interaction, which gives access to binding kinetic parameters from real time acquisition. It offers the possibility to test in a single run a large number of interactions, allowing rapid identification of the most suitable compounds toward a given biological entity. Until now, this technique has proven to be relevant for interaction between relatively large molecules (protein, antibodies, DNA) but has not been challenged yet for the screening of small molecules that can be of interested in the field of drug discovery. As a proof a principle, we have used SPRi to screen for interaction of several small molecules, referred to as G4-ligands on G-quadruplex DNA. This technology allowed to easy discrimination of the binding properties of four G4-ligands on quadruplex DNA models. (C) 2011 Elsevier B.V. All rights reserved.
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