期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 156, 期 1, 页码 410-415出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2011.04.066
关键词
Zinc; Fluorescent sensor; Two photon; Intracellular imaging
资金
- Chinese Academy of Sciences
- National Natural Science Foundation of China [90813002]
- National Basic Research Program of China [2011CB935800]
- Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, Central China Normal University
We report two fluorescent Zn2+ sensors, AD1 and AD2. In aqueous solution, upon addition of Zn2+. AD1 only showed the enhancement on the fluorescence intensity, while AD2, acting as a ratiometric sensor, demonstrated the variations not only on the fluorescence intensity but also on the emission wavelengths. Both sensors exhibit high sensitivity (nM level) and selectivity for Zn2+ over Na+, K+, Mg2+, and Ca2+ at the millimolar level. Moreover, AD2 undergoes significant increase of the TPA cross sections (delta(max)) at 840 nm by the Zn2+-coordination induced ICT enhancement. The laser scanning confocal microscopy experiments revealed that AD2 is cell-permeable and can indeed visualize the changes of intracellular Zn2+ in living cells through a ratiometric approach by utilizing two-photon excitation. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.
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