期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 160, 期 1, 页码 222-226出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2011.07.038
关键词
Biosensors; Phytase; Polypyrrole; Phytic acid detection
资金
- FAPESP
- CAPES
- CNPq (Brazil)
Amperometric biosensors based on the physical immobilization of phytase (PhyA) into polypyrrole (PPy) films were prepared in aqueous medium. The PPy/PhyA films were characterized by cyclic voltammetry, and surface and structural characterization techniques, SEM and FTIR. Both voltammetric and amperometric transduction methods were used in order to detect phytic acid in acetate buffer at pH 5.5 at room temperature. The biosensors exhibited a detection limit of 0.15 mmol L(-1) and a linear range of phytic acid content from 0.5 to 2.0 mmol L(-1), which are adequate values for typical analyses of phytic acids in most seeds, grains, and vegetables. (C) 2011 Elsevier B.V. All rights reserved.
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