Biomonitoring of methomyl pesticide by laccase inhibition on sensor containing platinum nanoparticles in ionic liquid phase supported in montmorillonite

This study focuses on the development and evaluation of a new biosensor for the determination of the pesticide methomyl, based on enzyme inhibition. Laccase (LAC) obtained from a genetically modified fungus (Aspergillus oryzae) was successfully immobilized in a new supported ionic liquid phase (SILP) based on platinum nanoparticles and the 1-butyl-3-methylimidazolium tetrafluoroborate ionic liquid (Pt-BMI.BF4) supported in montmorillonite. and subsequently applied in the construction of the biosensor. The process of inhibition by methomyl carbamate was performed using dopamine as a phenolic substrate to obtain the base signal. All measurements for the optimization and application of the biosensor were performed by square-wave voltammetry, and the best experimental conditions were obtained in acetate buffer solution (0.1 mol L-1, pH 5.5), with 0.5 units of enzyme and voltammetric parameters: 60 Hz of frequency, 100 mV of pulse amplitude and 8 mV of scan increment. The determination of methomyl in carrot and tomato samples using the proposed biosensor showed results consistent with those obtained by HPLC, verifying that the method developed can be used for the quantification of this pesticide. (C) 2011 Elsevier B.V. All rights reserved.