期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 156, 期 1, 页码 71-78出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2011.03.074
关键词
Electrochemical immunosensor; CS-Fe3O4-PAMAM-GNPs nanocomposites; HRP-MWCNTs-Ab bioconjugates; Signal amplification; Salbutamol detection
资金
- National Natural Science Foundation of the People's Republic of China [30972056, 30911140278]
- Foundation for Outstanding YoungScientist in Shandong Province [BS2009NY001]
- Scientific and Technological Development Plan in Shandong Province [2010GNC10960]
- Natural Science Foundation of Shandong Province [ZR2010DQ025]
- Shandong Province Higher Educational Science and Technology [J10LB14]
An ultrasensitive electrochemical immunosensor based on chitosan-iron oxide-poly(amino-amine) dendrimers-gold nanoparticles (CS-Fe3O4-PAMAM-GNPs) nanocomposites and horseradish peroxidase-multiwall carbon nanotubes-antibody (HRP-MWCNTs-Ab) bioconjugates was developed for the detection of salbutamol (SAL). CS-Fe3O4-PAMAM-GNPs nanocomposites as immobilization matrix were used to enhance the electroactivity and stability of the electrode. HRP-MWCNTs-Ab bioconjugates as label were used to improve catalytic activity for hydrogen reduction of the electrode. Under the optimized conditions, a calibration plot for SAL was obtained with a linear range between 0.11 ng/mL and 1061 ng/mL (r = 0.9984). The detection limit was 0.06 ng/mL. The immunosensor was examined in real samples for the analysis of SAL. (C) 2011 Elsevier B.V. All rights reserved.
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