期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 145, 期 1, 页码 444-450出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2009.12.055
关键词
Horseradish peroxidase; Biosensor; Redox polymer; Mediator; Ferrocene
资金
- T.R. Prime Ministry State Planning Organization
- Fatih University Research Support Office [P50090801-1]
In this work, a new type of amperometric hydrogen peroxide biosensor was fabricated for the determination of H2O2. Horseradish peroxidase (HRP) was immobilized on a glassy carbon electrode by poly(glycidyl methacrylate-co-vinylferrocene) (poly(GMA-co-VFc)) film. A polymeric electron transfer mediator, containing copolymers of glycidyl methacrylate (GMA) and vinylferrocene (VFc) with different molar ratios, was prepared by free-radical copolymerization. The amperometric response was measured as a function of H2O2 concentration, at a fixed potential of +0.35 V vs. Ag/AgCl in phosphate-buffered saline (pH 7.0). The mediated hydrogen peroxide biosensor showed a fast response of less than 4s of linear range 2.0-30.0 mM, with a detection of 2.6 mu M. The sensitivity of the biosensor for H2O2 was 10.42 nA/mM cm(2). (C) 2009 Elsevier B.V. All rights reserved.
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