期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 143, 期 2, 页码 681-688出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2009.10.028
关键词
Shape-coded; Hydrogel microparticles; Microfluidic device; Multiplexed enzyme-based assay; Photopatterning
资金
- MEST [R11-2007-050-03002-0, R01-2007-000-11495-0]
- Seoul Research and Business Development Program [NT080584]
- National Research Foundation of Korea [2007-0053817, R11-2007-050-03002-0, R01-2007-000-11495-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This paper describes a multiplexed enzyme-based assay within a microfluidic device using shape-coded poly(ethylene glycol) (PEG) hydrogel microparticles. A microfluidic device was constructed by serially connecting two patterning chambers and a microfilter-integrated detection chamber through a Y-shaped microchannel. Various shapes and sizes of hydrogel microparticles entrapping different enzymes were fabricated in the patterning chamber by photolithography and collected in the detection chamber by pressure-driven flow. Due to the action of the microfilter, hydrogel microparticles were perfectly retained inside the detection chamber without passing through it. The sequential bienzymatic reactions of glucose oxidase (GOX) and peroxidase (POD) or alcohol oxidase (AOX) and POD were successfully investigated using Amplex Red within the hydrogel microparticles. Furthermore, by entrapping GOX and AOX into different shapes of hydrogel microparticles, each enzyme-catalyzed reaction was easily identified, and therefore, simultaneous detection of glucose and ethanol was possible in the concentration range from 1.0 to 10.0 mM without cross-talk by using same fluorescence indicator in a single detection chamber. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据