期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 138, 期 1, 页码 344-350出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2009.01.002
关键词
Glucose biosensor; ZnO; Nanotubes; Glucose oxidase; Electrochemical method
资金
- National Key Basic Research Program [2006CB922002]
- Natural Science Foundation of China [50721091, 10874165, 50532040]
A glucose biosensor is fabricated with immobilization of glucose oxidase onto ZnO nanotube arrays by cross-linking method. The ZnO nanotube arrays are synthesized by chemical etching of ZnO nanorods that are electrochemically deposited on the Au surface. Morphology and structure of ZnO nanotubes are characterized by FESEM, HRTEM and XRD. Fourier-trans form infrared spectroscopy reveals that the glucose oxidase immobilized on the ZnO nanotubes retains its native conformation. The biosensor has a wide linear range for the detection of glucose from 50 mu M to 12 mM (a correlation coefficient of 0.998) with 3s response time. The sensitivity of the biosensor is found to be 21.7 mu A/mM cm(2). Moreover, its experimental detection limit is I mu M (S/N = 3) and the apparent Michaelis-Men ten constant is calculated to be 19 mM. The anti-interference ability and long-term stability of the biosensor are also assessed. Compared with the biosensors based on the nanorod and flat structure, the proposed biosensor shows expanded linear range and sensitivity. All these results demonstrate that ZnO nanotube can provide a promising material for the biosensor designs and other biological applications. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据