期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 136, 期 2, 页码 510-515出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2008.10.012
关键词
Xanthine oxidase; Horseradish peroxidase; Calcium carbonate; Nanoparticles; Xanthine; Biosensor
资金
- National Natural Science Foundation of China [20505014, 20773108]
- Chinese Ministry of Education [207041]
Using calcium carbonate nanoparticles as enzyme immobilization matrix, the developments of xanthine biosensor were achieved by both electrooxidation and electroreduction of the enzymatic-generated hydrogen peroxide based on xanthine oxidase (XnOx) and horseradish peroxidase (HRP). Amperometric detection of xanthine was evaluated by holding the modified electrode at 0.55 and -0.05 V (versus SCE), for XnOx/Nano-CaCO(3) and XnOx/HRP/Nano-CaCO(3), respectively. The linear dynamic ranges of anodic and cathodic detections of xanthine were between 2 x 10(-6) to 2.5 x 10(-4) M and 4 x 10(-7) to 5 x 10(-5) M, respectively. The detection limits were determined to be of 2 x 10(-6) and 1 x 10(-7) M with anodic and cathodic processes, respectively. At lower working potential, XnOx/HRP/Nano-CaCO(3)/GCE bienzymatic system exhibited excellent selectivity: the bienzyme electrode was inert towards ascorbic and uric acid present. Moreover, the permeability of enzyme/Nano-CaCO(3) was evaluated by the use of rotating disk electrode voltammetry. (C) 2008 Elsevier B.V. All rights reserved.
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