期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 138, 期 1, 页码 244-250出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2008.12.009
关键词
Hemoglobin; Amphiphilic block copolymer; Direct electrochemistry; Nitric oxide; Electrocatalysis
资金
- Key Project of Chinese Ministry of Education [206104]
- National Natural Science Foundation of China [20674064, 20874081]
- Open Project Program of Key Laboratory of Environmentally Friendly Chemistry and Applications (Xiangtan University)
- Ministry of Education, China
- Multidiscipline Scientific Research Foundation of Xiangtan University [051ND08]
A well-defined amphiphilic diblock copolymer, poly [N-(2-methacryloyloxyethyl) pyrrolidone]-block-poly [glycidyl methacrylate] (PNMP-b-PGMA), was applied to immobilize hemoglobin (Hb) in a simple procedure. The characterization of PNMP-b-PGMA/Hb film was demonstrated by ultraviolet-visible (UV-vis) spectra, atomic force microscopy (AFM), and electrochemical impedance spectroscopy (EIS). The electrochemical behaviors of Hb in PNMP-b-PGMA film have been investigated and a pair of well-defined and nearly reversible cyclic voltammetric peaks for the protein heme Fe(III)/Fe(II) redox couples were observed at about -0.409V (vs. SCE) in pH 7.0 phosphate buffer solution (PBS). The results showed that the PNMP-b-PGMA film improved the protein loading with the retention of bioactivity and greatly promoted the direct electron transfer between Hb and electrode surface. This PNMP-b-PGMA/Hb film modified electrode also exhibited high electrocatalytic effects towards reduction of nitric oxide (NO). These results indicated the potential applicability of the films as new types of third-generation biosensors or bioreactors based on direct electrochemistry of the proteins. (C) 2008 Elsevier B.V. All rights reserved.
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