期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 141, 期 1, 页码 13-19出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2009.06.007
关键词
Optical biosensors; Photonic crystal microcavity; Reaction kinetics; Affinity constant
We report on time-resolved label-free monitoring of protein binding in a physiological buffer using a photonic crystal microcavity sensor of total area 50 mu m(2) with an effective detection area of 0.272 mu m(2). We use this ultracompact sensor to monitor the binding of anti-biotin to biotinylated-bovine serum albumin (b-BSA), and measure an affinity constant of 6.94 x 10(7) M(-1). We show that this photonic crystal sensor can be used for anti-biotin detection at concentrations ranging from picomolar to micromolar. The lower limit of detection for anti-biotin is less than 20 pM. corresponding to less than 4.5 fg of bound material on the sensor surface and fewer than 80 molecules in the modal volume of the microcavity. The sensor also has the capability of measuring binding of small molecular species such as aromatic rings (98 Da). Furthermore, we show that the active surface of the sensor can be successfully regenerated and re-used in subsequent protein binding experiments. A comparison of experimental and theoretical data is given, and the current experimental limitations of the sensor with regard to noise are discussed. (C) 2009 Elsevier B.V. All rights reserved.
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