期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 142, 期 1, 页码 267-272出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2009.08.013
关键词
Myoglobin; KIT-6; Direct electron transfer; Screen-printed electrode; Biosensor
资金
- Shanghai Nano-Technology Promotion Center (SNPC) [0752nm025]
- Ministry of Science and Technology of the People's Republic of China [0424]
disposable hydrogen peroxide biosensor was developed based on the direct electron transfer of myoglobin (Mb) on mesopores KIT-6 modified screen-printed electrode (SPE) which was manually performed to fabricate the planar carbon electrodes. KIT-6 is a new material which can absorb abundant of Mb molecules. A mixture of Mb and KIT-6 was immobilized with nafion on electrode. The cyclic voltammetry experiment indicated that a pair of stable and well-defined reduction peaks with a formal potentials of -0.35, and -0.28V versus saturated calomel electrode (SCE) was obtained, using the present modified electrode in phosphate buffer saline (0.05 M, pH 7.0) at scan rate of 100mV s(-1), characteristic of Mb heme Fe(III)/Fe(II) redox couple. The heterogeneous electron transfer rate constant k(s) was estimated to be 16.93 s(-1). And the formal potential was pH-dependent, having two slopes of -54.7 and -49.3 mV/pH which illustrated one electron transfer. This modified electrode was applied to detect H2O2 with sensitivity of 55.68 mAM(-1) cm(-2). Infrared spectrum and UV-vis absorption spectra of immobilized Mb film were recorded. In conclusion, KIT-6 increases the electron transfer activity of Mb and this kind of H2O2 biosensor is low cost for using disposable. (C) 2009 Elsevier B. V. All rights reserved.
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