期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 139, 期 1, 页码 118-124出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2008.10.001
关键词
Water soluble porphyrin; Bovine serum albumin; Time resolved fluorescence; Intrinsic fluorescence
The interaction of meso-tetrakis(p-sulfonatophenyl)porphyrin (TPPS) with bovine serum albumin (BSA) in neutral solution (pH 7.4) has been studied by means of absorption. steady-state fluorescence and time-resolved fluorescence spectroscopy. The formation of TPPS-BSA complex was monitored by spectroscopic characteristic changes in Soret band absorption and fluorescence emission of TPPS. Applying the time-correlated single-photon counting (TCSPC) method, BSA-TPPS interaction was also investigated by the fluorescence lifetime of tryptophan residues in BSA. The results demonstrate that deep UV laser-based fluorescence lifetime microscopy is useful for sensitive identification of protein interaction using intrinsic fluorescence. (C) 2008 Elsevier B.V. All rights reserved.
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