期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 138, 期 2, 页码 532-538出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2009.02.060
关键词
Biosensors; Bioassays; Quartz crystal microbalance; Biological warfare agents
资金
- Mission for Research and Scientific Innovation of French Delegation for Armament and Weaponry (DGA/MRIS) [06.34.025]
This paper focuses on flow functionalization of piezoelectric immunochips with antibodies against four different biological warfare agents (BWA) surrogates. To perform parallel detection of all BWA surrogates at once, the E4 Quartz Crystal Microbalance with Dissipation monitoring system (QCM-D) is used. Assessment of antibodies immobilization, parallel detection of related BWA surrogates diluted in buffer solutions and regeneration of the complex antibodies/BWA surrogates are first discussed. Minimal detection thresholds for Escherichia coli MRE 162, Bacillus atrophaeus, Cydia pomonella granulosis virus (CpGV) and ovalbumin are respectively equal to 2.4 x 10(7) CFU/mL. 1.4 x 10(6) spores/mL, 1.1 x 10(8) granules/mL and 1 mu g/mL. Detection experiments for three of the four BWA surrogates (E. coli MRE 162, B. atrophaeus and ovalbumin) immersed in real liquid matrices from air sampler are successfully performed. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据