期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 138, 期 2, 页码 491-496出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2009.02.058
关键词
Biosensor; BVDV; Static light scattering; Immunoassay; Virus detection; Lab-on-a-chip
资金
- Agriculture & Forestry Technology Management Center, Republic of Korea [C-AD14-2006-11-00] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
We have investigated the utilization of microparticle immunoagglutination assays using forward light scattering measurements in a microfluidic chip towards detecting viral particles. The model viral target was bovine viral diarrhea Virus (BVDV). Highly carboxylated polystyrene microparticles (510 nm) were coated with anti-BVDV monoclonal antibodies. This solution Was in turn used to detect BVDV in diluted tissue culture media and fetal calf serum. The assay was first performed in a two well slide format for proof of concept and particles stability experiments, then in a simple y-channel microfluidic chip with optical fibers arranged in a close proximity setup. Microparticle immunoagglutination was detected via static forward light scattering measurements taken at 45 to incident light. In the microfluidic chip, BVDV was detected down to a concentration of 10 TCID50 mL(-1). For assay comparison purposes reverse transcriptase polymerase chain reaction (RT-PCR) was performed Oil serial dilutions of the BVDV sample used in the two well and microfluidic testing. RT-PCR was effective clown to a concentration of 10(3) TCID50 mL(-1) in detecting the identical BVDV used for microfluidic assays. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据