期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 129, 期 1, 页码 372-379出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2007.08.034
关键词
aptamer; biosensor; electrochemical impedance spectroscopy; MEMS; pyrolyzed carbon; thrombin
资金
- Ministry of Education, Science & Technology (MoST), Republic of Korea [K08007] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [과06A1506] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
We present an electrochemical impedance biosensor utilizing pyrolyzed carbon film as a working electrode material for aptamer-based thrombin detection. Batch-fabricated, smooth thin film carbon electrodes, which are fabricated by photolithography and photoresist thermal decomposition at high temperatures in inert ambient, are obtained for integrated electrochemical biosensors. To confirm the suitability of pyrolyzed carbon for use in an electrochemical biosensor, physical and electrical properties of carbon film pyrolyzed with a positive photoresist, AZ9260, were studied. Film thickness after pyrolysis was between 19% and 15% relative to the initial photoresist thickness and the cross-section was changed from rectangular to round shape due to the photoresist reflow characteristics. Resistivity of carbon thin film pyrolyzed at 1000 degrees C was 3 m Omega cm, which is comparable to that of highly boron-doped polysilicon. The pyrolysis temperature of 1000 degrees C was chosen in order to obtain carbon film with high conductivity for use as a working electrode. Thrombin aptamer was grafted onto the pyrolyzed carbon surface using carbodiimide-mediated chemistry, followed by Triton-X 100 and BSA treatment to reduce non-specific binding of thrombin. Electron-transfer resistance changes due to thrombin binding onto the carbon surface were measured using electrochemical impedance spectroscopy techniques. Thrombin concentrations between 0.5 nM and 500 nM were detected by electrochemical measurement. Pyrolyzed carbon can provide a new approach for miniaturization, integration, and low-cost fabrication in electrochemical biosensors. (C) 2007 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据